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If cleansing the sensor doesn’t take care of the situation, change the sensor and assure its positioning is accurate.First, we ended up impressed with technician Dan who termed forward to let us know when he’d be in this article. Wonderful! He spent time in outlining the trouble, replaced the rollers and lubed all the things. In 6 hrs from star
This really is especially accurate for immunohistochemistry during which ten% NBF is proven for being a weak fixative (Fig. 2). Some issues with using ten% NBF being a fixative are corrected from the introduction of other experimental tactics for instance antigen retrieval (Shi et al. 2001) and the usage of small amplicons in RT-Q-PCR (Steg et al.
The incubation time in liquid paraffin need to be prolonged adequate to get satisfactory infiltration, but not any more than essential in an effort to avert extended publicity from the tissue to heat. For the same explanation, it is usually recommended which the temperature of the liquid paraffin ought to be not more than two–4 C higher than the
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